Actions of Insect Toxin and Other Toxins Derived from the Venom of the Scorpion Androctonus Austraus on Isolated Giant Axons

1. Insect toxin, mammal toxins I and II and crustacean toxin were obtained from the venom of the scorpion Androctonus australis. Their effects on the isolated giant axon of the cockroach Periplaneta americana were investigated by current-clamp and voltage-clamp techniques. 2. In current-clamp conditions, mammal toxins and crustacean toxin (1*3—13 /IM) induced a large prolongation of the falling phase of the evoked action potentials. Insect toxin (0-13-3-3 A) induced a progressive slow depolarization of the membrane potential and repetitive firing of action potentials. No changes in the time-course of the action potential were induced by insect toxin. 3. In voltage-clamp conditions, mammal and crustacean toxins induced a slowing of the turn-off of the transient inward sodium current, with either no change or a small increase in the peak sodium current. Insect toxin by contrast induced an increase in the peak sodium current and a slowing of the sodium current turn-off, this effect being greatest at lower values of the clamped membrane voltage. 4. It is concluded that the repetitive activity induced by insect toxin results from a voltage-dependent modulation of sodium inactivation coupled with an increase in both the resting and active sodium permeabilities of the cockroach axonal membrane.